TSG101 antibody (AA 250-330)

Catalog No. ABIN7224820

The Rabbit Polyclonal anti-TSG101 antibody is suitable to detect TSG101 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for TSG101 antibody (AA 250-330) (ABIN7224820)

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TSG101 Antibody

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-Tsg 101 Polyclonal Antibody

Specificity: Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: Tsg 101

Background: TSG101, Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101The protein encoded by TSG101 (tumor susceptibility 101) belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in TSG101 occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Molecular Weight: 45kD

Gene ID: 7251

UniProt: Q99816