TNK2 antibody (pTyr284)

Catalog No. ABIN7218908

The Rabbit Polyclonal anti-TNK2 antibody is suitable to detect TNK2 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for TNK2 antibody (pTyr284) (ABIN7218908)

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TNK2 Antibody

Binding Specificity: pTyr284

Purpose: Rabbit Anti-ACK (phospho Tyr284) Polyclonal Antibody

Specificity: Phospho-ACK (Y284) Polyclonal Antibody detects endogenous levels of ACK protein only when phosphorylated at Y284.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ACK Phospho-Tyr284

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TNK2

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Alternative Name: ACK

Background: TNK2, ACK1, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2TNK2 encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The tyrosine kinase non receptor 2 may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Molecular Weight: 120kD

Gene ID: 10188

UniProt: Q07912