TNK2 antibody

Catalog No. ABIN7218910

The Rabbit Polyclonal anti-TNK2 antibody (ABIN7218910) specifically detects TNK2 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for TNK2 antibody (ABIN7218910)

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TNK2 Antibody

Purpose: Rabbit Anti-ACK Polyclonal Antibody

Specificity: ACK Polyclonal Antibody detects endogenous levels of ACK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ACK around the non-phosphorylation site of Y284

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TNK2

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Alternative Name: ACK

Background: TNK2, ACK1, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2TNK2 encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The tyrosine kinase non receptor 2 may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc43Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Molecular Weight: 115kD

Gene ID: 10188

UniProt: Q07912