PRAGMIN antibody (pTyr413)

Catalog No. ABIN7219922

This Rabbit Polyclonal antibody specifically detects PRAGMIN in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRAGMIN antibody (pTyr413) (ABIN7219922)

Target: PRAGMIN (SGK223) (Tyrosine-Protein Kinase SgK223 (SGK223))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAGMIN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PRAGMIN Antibody

Binding Specificity: pTyr413

Purpose: Rabbit Anti-Pragmin (phospho Tyr413) Polyclonal Antibody

Specificity: Phospho-Pragmin (Y413) Polyclonal Antibody detects endogenous levels of Pragmin protein only when phosphorylated at Y413.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Pragmin Phospho-Tyr413

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PRAGMIN

Target: PRAGMIN (SGK223) (Tyrosine-Protein Kinase SgK223 (SGK223))

Alternative Name: Pragmin

Background: SGK223, Tyrosine-protein kinase SgK223, Sugen kinase 223SGK223 encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA).

Molecular Weight: 175kD

Gene ID: 157285

UniProt: Q86YV5