U2AF1 antibody (AA 60-140)

Catalog No. ABIN7231978

This Rabbit Polyclonal antibody specifically detects U2AF1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for U2AF1 antibody (AA 60-140) (ABIN7231978)

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-U2AF1 Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-U2AF1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of U2AF1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human U2AF1 protein at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for U2AF1

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Alternative Name: U2AF1

Background: Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunitU2AF1 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF1 encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 36kD

Gene ID: 7307

UniProt: Q01081