USP11 antibody (Internal Region)

Catalog No. ABIN7218842

The Rabbit Polyclonal anti-USP11 antibody is suitable to detect USP11 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for USP11 antibody (Internal Region) (ABIN7218842)

Target: USP11 (Ubiquitin Specific Peptidase 11 (USP11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-USP11 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-USP11 Polyclonal Antibody

Specificity: USP11 Polyclonal Antibody detects endogenous levels of USP11 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human USP11

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for USP11

Target: USP11 (Ubiquitin Specific Peptidase 11 (USP11))

Alternative Name: USP11

Background: USP11, UHX1, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11USP11 (Ubiquitin Specific Peptidase 11) is a Protein Coding gene. Among its related pathways are Transport to the Golgi and subsequent modification and Chaperonin-mediated protein folding. GO annotations related to this gene include cysteine-type endopeptidase activity and thiol-dependent ubiquitinyl hydrolase activity. An important paralog of this gene is USP4. rotease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed: 12084015, PubMed: 15314155, PubMed: 17897950, PubMed: 19874889, PubMed: 20233726, PubMed: 24724799). Inhibits the degradation of target proteins by the proteasome (PubMed: 12084015). Cleaves preferentially Lys-6 and Lys-63-linked ubiquitin chains. Has lower activity with Lys-11 and Lys-33-linked ubiquitin chains, and extremely low activity with Lys-27, Lys-29 and Lys-48-linked ubiquitin chains (in vitro) (PubMed: 24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed: 17897950, PubMed: 19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed: 15314155, PubMed: 20233726).

Molecular Weight: 105kD

UniProt: P51784