USP45 antibody (AA 270-350)

Catalog No. ABIN7225498

The Rabbit Polyclonal anti-USP45 antibody is suitable to detect USP45 in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for USP45 antibody (AA 270-350) (ABIN7225498)

Target: USP45 (Ubiquitin Specific Peptidase 45 (USP45))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP45 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-USP45 Antibody

Binding Specificity: AA 270-350

Purpose: Rabbit Anti-USP45 Polyclonal Antibody

Specificity: USP45 Polyclonal Antibody detects endogenous levels of USP45 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human USP45 at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for USP45

Target: USP45 (Ubiquitin Specific Peptidase 45 (USP45))

Alternative Name: USP45

Background: USP45, Ubiquitin carboxyl-terminal hydrolase 45, Deubiquitinating enzyme 45, Ubiquitin thioesterase 45, Ubiquitin-specific-processing protease 45The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. A wide range of enzymes facilitate the proteolytic Ub pathway, including USPs (ubiquitin specific peptidases), which are cysteine proteases that are responsible for the release of ubiquitin from a ubiquitylated substrate and precursor fusion proteins. USP45 (Ubiquitin carboxyl-terminal hydrolase 45), also known as Deubiquitinating enzyme 45, is a 819 amino acid protein that is involved in ubiquitin-dependent protein catabolism. USP45 differs from other USPs in that it contains a UBP-type zinc finger, a domain which binds ubiquitin. Although USP45 is broadly expressed, the highest levels can be found in skeletal muscle, spleen and ovary. There are three isoforms of USP45 which are produced as a result of alternative splicing.

Molecular Weight: 90kD

Gene ID: 85015

UniProt: Q70EL2