UBE2E3 antibody

Catalog No. ABIN7231982

The Rabbit Polyclonal anti-UBE2E3 antibody (ABIN7231982) specifically detects UBE2E3 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for UBE2E3 antibody (ABIN7231982)

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBE2E3 Antibody

Purpose: Rabbit Anti-UB2E3 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of UB2E3 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human UB2E3 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBE2E3

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: UB2E3

Background: Ubiquitin-conjugating enzyme E2 E3, E2 ubiquitin-conjugating enzyme E3, UbcH9, Ubiquitin carrier protein E3, Ubiquitin-conjugating enzyme E2-23 kDa, Ubiquitin-protein ligase E3UBE2E3 (Ubiquitin Conjugating Enzyme E2 E3) is a Protein Coding gene. Among its related pathways are Protein ubiquitination and Class I MHC mediated antigen processing and presentation. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2E3 encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for UBE2E3.

Molecular Weight: 22kD

Gene ID: 10477

UniProt: Q969T4