UBE4A antibody (N-Term)

Catalog No. ABIN7217712

The Rabbit Polyclonal anti-UBE4A antibody (ABIN7217712) specifically detects UBE4A in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBE4A antibody (N-Term) (ABIN7217712)

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-UBE4A Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-UBE4A Polyclonal Antibody

Specificity: UBE4A Polyclonal Antibody detects endogenous levels of UBE4A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human UBE4A

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBE4A

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Alternative Name: UBE4A

Background: UBE4A, KIAA0126, Ubiquitin conjugation factor E4 AUBE4A encodes a member of the U-box ubiquitin ligase family. The ubiquitination factor E4A is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 122kD

Gene ID: 9354

UniProt: Q14139