UCP3 antibody (C-Term)

Catalog No. ABIN7217440

The Rabbit Polyclonal anti-UCP3 antibody (ABIN7217440) specifically detects UCP3 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UCP3 antibody (C-Term) (ABIN7217440)

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UCP3 Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-UCP3 Polyclonal Antibody

Specificity: UCP3 Polyclonal Antibody detects endogenous levels of UCP3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human UCP3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UCP3

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Alternative Name: UCP3

Background: UCP3, SLC25A9, Mitochondrial uncoupling protein 3, UCP 3, Solute carrier family 25 member 9Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression, this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.

Molecular Weight: 33kD

Gene ID: 7352

UniProt: P55916

Pathways: Proton Transport