UBTF antibody

Catalog No. ABIN7224858

The Rabbit Polyclonal anti-UBTF antibody has been validated for WB and ELISA. It is suitable to detect UBTF in samples from Human, Mouse and Rat.

Quick Overview for UBTF antibody (ABIN7224858)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBTF Antibody

Purpose: UBF Polyclonal Antibody

Specificity: UBF Polyclonal Antibody detects endogenous levels of UBF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human UBF around the non-phosphorylation site of S484

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBF

Background: Rabbit Anti-UBF Polyclonal Antibody,UBTF, UBF, UBF1, Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1,UBTF (upstream binding transcription factor, RNA polymerase I) encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for UBTF. Pseudogenes of UBTF are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11.,Nucleolar transcription factor 1

Molecular Weight: observerd band 89kDa

Gene ID: 7343

UniProt: P17480