MYB antibody (pSer12)

Catalog No. ABIN7222578

The Rabbit Polyclonal anti-MYB antibody (ABIN7222578) specifically detects MYB in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for MYB antibody (pSer12) (ABIN7222578)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MYB Antibody

Binding Specificity: pSer12

Purpose: Rabbit Anti-c-Myb (phospho Ser12) Polyclonal Antibody

Specificity: Phospho-c-Myb (S12) Polyclonal Antibody detects endogenous levels of c-Myb protein only when phosphorylated at S12.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human c-Myb Phospho-Ser12

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: c-Myb

Background: MYB, Transcriptional activator Myb, Proto-oncogene c-MybMYB encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. MYB may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants.

Molecular Weight: 65kD

Gene ID: 4602

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process