V-ATPase D1 antibody (Internal Region)

Catalog No. ABIN7217604

The Rabbit Polyclonal anti-V-ATPase D1 antibody (ABIN7217604) specifically detects V-ATPase D1 in ELISA and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for V-ATPase D1 antibody (Internal Region) (ABIN7217604)

Target: V-ATPase D1 (VHA36-1) (Vacuolar H[+] ATPase Subunit 36-1 (VHA36-1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V-ATPase D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-V-ATPase D1 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-V-ATPase D1 Polyclonal Antibody

Specificity: V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human V-ATPase D1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for V-ATPase D1

Target: V-ATPase D1 (VHA36-1) (Vacuolar H[+] ATPase Subunit 36-1 (VHA36-1))

Alternative Name: V-ATPase D1

Background: ATP6V0D1, ATP6D, VPATPD, V-type proton ATPase subunit d 1, V-ATPase subunit d 1, 32 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, p39, Vacuolar proton pump subunit d 1ATP6V0D1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

Molecular Weight: 40kD

Gene ID: 9114

UniProt: P61421