VCP antibody

Catalog No. ABIN7224896

The Rabbit Polyclonal anti-VCP antibody is suitable to detect VCP in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for VCP antibody (ABIN7224896)

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-VCP Antibody

Purpose: Rabbit Anti-VCP Polyclonal Antibody

Specificity: VCP Polyclonal Antibody detects endogenous levels of VCP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human VCP around the non-phosphorylation site of S352

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: VCP

Background: VCP, Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCPThe protein encoded by VCP (valosin containing protein) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Molecular Weight: 85kD

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway