VAV3 antibody (pTyr173)

Catalog No. ABIN7218990

The Rabbit Polyclonal anti-VAV3 antibody is suitable to detect VAV3 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for VAV3 antibody (pTyr173) (ABIN7218990)

Target: VAV3 (Vav 3 Oncogene (VAV3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAV3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-VAV3 Antibody

Binding Specificity: pTyr173

Purpose: Rabbit Anti-Vav3 (phospho Tyr173) Polyclonal Antibody

Specificity: Phospho-Vav3 (Y173) Polyclonal Antibody detects endogenous levels of Vav3 protein only when phosphorylated at Y173.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Vav3 Phospho-Tyr173

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: Vav3

Background: VAV3, Guanine nucleotide exchange factor VAV3, VAV-3VAV3 is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 100kD

Gene ID: 10451

UniProt: Q9UKW4

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway