Vimentin antibody (pTyr61)

Catalog No. ABIN7224922

This Rabbit Polyclonal antibody specifically detects Vimentin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Vimentin antibody (pTyr61) (ABIN7224922)

Target: Vimentin (VIM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Vimentin Antibody

Binding Specificity: pTyr61

Purpose: Vimentin (phospho Tyr61) Polyclonal Antibody

Specificity: Phospho-Vimentin (Y61) Polyclonal Antibody detects endogenous levels of Vimentin protein only when phosphorylated at Y61.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Vimentin Phospho-Tyr61

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Rabbit Anti-Vimentin (phospho Tyr61) Polyclonal Antibody,VIM, Vimentin,VIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.,Vimentin

Molecular Weight: observerd band 57kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis