Vimentin antibody (AA 20-90)

Catalog No. ABIN7226806

This Rabbit Polyclonal antibody specifically detects Vimentin in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Vimentin antibody (AA 20-90) (ABIN7226806)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Vimentin Antibody

Binding Specificity: AA 20-90

Purpose: Vimentin (Phospho-Tyr38) Polyclonal Antibody

Specificity: The antibody detects endogenous Vimentin (Phospho-Tyr38) protein.

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthetic peptide from human protein at AA range: 20-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:10000), ELISA (1:10000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Rabbit Anti-Vimentin (Phospho-Tyr38) Polyclonal Antibody,VIM,VIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.,vimentin

Molecular Weight: observerd band 51kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis