Vitamin D Receptor antibody (pSer51)

Catalog No. ABIN7224898

This Rabbit Polyclonal antibody specifically detects Vitamin D Receptor in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Vitamin D Receptor antibody (pSer51) (ABIN7224898)

Target: Vitamin D Receptor (VDR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Vitamin D Receptor Antibody

Binding Specificity: pSer51

Purpose: Rabbit Anti-VDR (phospho Ser51) Polyclonal Antibody

Specificity: Phospho-VDR (S51) Polyclonal Antibody detects endogenous levels of VDR protein only when phosphorylated at S51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human VDR Phospho-Ser51

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: VDR

Target Type: Chemical

Background: VDR, NR1I1, Vitamin D3 receptor, VDR, 1, 25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1VDR (vitamin D (1,25- dihydroxyvitamin D3) receptor) encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in VDR are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins.

Molecular Weight: 38kD

Gene ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway