WTAP antibody (AA 290-370)

Catalog No. ABIN7217508

The Rabbit Polyclonal anti-WTAP antibody is suitable to detect WTAP in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for WTAP antibody (AA 290-370) (ABIN7217508)

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-WTAP Antibody

Binding Specificity: AA 290-370

Purpose: Rabbit Anti-WTAP Polyclonal Antibody

Specificity: WTAP Polyclonal Antibody detects endogenous levels of WTAP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human WTAP at AA range: 290-370

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP

Background: WTAP, KIAA0105, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, hFL(2)D, WT1-associated protein, Wilms tumor 1-associating proteinThe Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. WTAP encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, pre-mRNA-splicing regulator WTAP is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.

Molecular Weight: 55kD

Gene ID: 9589

UniProt: Q15007