WNK1 antibody (pThr60)

Catalog No. ABIN7213256

The Rabbit Polyclonal anti-WNK1 antibody (ABIN7213256) specifically detects WNK1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for WNK1 antibody (pThr60) (ABIN7213256)

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-WNK1 Antibody

Binding Specificity: pThr60

Purpose: Rabbit Anti-WNK1 (phospho Thr60) Polyclonal Antibody

Specificity: Phospho-WNK1 (T60) Polyclonal Antibody detects endogenous levels of WNK1 protein only when phosphorylated at T60.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human WNK1 Phospho-Thr60

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Alternative Name: WNK1

Background: WNK1, HSN2, KDP, KIAA0344, PRKWNK1, Serine/threonine-protein kinase WNK1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1WNK1 encodes a member of the WNK subfamily of serine/threonine protein kinases. WNK lysine deficient protein kinase 1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in WNK1 have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.

Molecular Weight: 230kD

Gene ID: 65125

UniProt: Q9H4A3