WWOX antibody (pTyr33)

Catalog No. ABIN7223100

The Rabbit Polyclonal anti-WWOX antibody (ABIN7223100) specifically detects WWOX in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for WWOX antibody (pTyr33) (ABIN7223100)

Target: WWOX (WW Domain Containing Oxidoreductase (WWOX))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWOX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-WWOX Antibody

Binding Specificity: pTyr33

Purpose: Rabbit Anti-WWOX (phospho Tyr33) Polyclonal Antibody

Specificity: Phospho-WWOX (Y33) Polyclonal Antibody detects endogenous levels of WWOX protein only when phosphorylated at Y33.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human WWOX Phospho-Tyr33

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for WWOX

Target: WWOX (WW Domain Containing Oxidoreductase (WWOX))

Alternative Name: WWOX

Background: WWOX, FOR, WOX1, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductaseWWOX (WW domain containing oxidoreductase) encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. WWOX spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of WWOX is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants.

Molecular Weight: 55kD

Gene ID: 51741

UniProt: Q96KM3