Lipopolysaccharides (LPS) antibody
Quick Overview for Lipopolysaccharides (LPS) antibody (ABIN7232847)
Target
See all Lipopolysaccharides (LPS) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-Legionella pneumophila Mouse Monoclonal Antibody
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Specificity
- This antibody recognizes LPS of L. pneumophila serogroup 1 (SG1). It does not react with serogroups 2-9, Legionella bozemanae, or Branhamella catarrharlis.
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Cross-Reactivity
- Legionella pneumophila
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Immunogen
- L. pneumophila serogroup 1 LPS conjugated to BSA.
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Isotype
- IgG1
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Application Notes
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ELISA: use at 0.1-1.0 μg/mL with LPS on the solid phase at 5 μg/mL.
These are recommended concentrations.
End users should determine optimal concentrations for their applications. -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, 0.02 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- This antibody is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
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- Lipopolysaccharides (LPS)
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Alternative Name
- LPS
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Target Type
- Chemical
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Background
- Legionella pneumophila are Gram- negative aerobic coccobacilli isolated from natural and artificial fresh water reservoirs. The outer membrane of L. pneumophila is comprised of a unique lipopolysaccharide (LPS) which is less endotoxic than enterobacterial LPS. Serogroups of L. pneumophila are classified based on O- antigen determinants of their cell wall side chains. L. pneumophila is pathogenic for humans, and it has no known soil or animal sources. It is responsible for more than 98 % of the cases of Legionnaires' Disease, and about 95 % of cases are due to serogroup 1. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, blood, and urine.
Target
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