GCET2 antibody

Catalog No. ABIN7232905

This Mouse Monoclonal antibody specifically detects GCET2 in IHC and WB. It exhibits reactivity toward Human.

Quick Overview for GCET2 antibody (ABIN7232905)

Target: GCET2 (Germinal Center Expressed Transcript 2 (GCET2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCET2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 1H1-A7

Product Details anti-GCET2 Antibody

Purpose: Anti-HGAL Mouse Monoclonal Antibody

Specificity: This antibody recognizes human HGAL.

Cross-Reactivity: Human

Immunogen: GST-HGAL fusion protein (Accession no. AF521911)

Isotype: IgG2a

Application Details

Application Notes:
Immunohistochemistry: use at 1-10 μg/mL on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10 mM, pH 6.0, 10 minutes).
Immunoblotting: use at 1-10 μg/mLHGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Target Details for GCET2

Target: GCET2 (Germinal Center Expressed Transcript 2 (GCET2))

Alternative Name: GCSAM

Background: Germinal center-associated signaling and motility protein,Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. {PubMed:17823310, PubMed:20844236, PubMed:23299888}.,The human germinal-center-associated lymphoma (HGAL) gene was originally identified from an expressed sequence tag that was associated with improved survival in patients with diffuse large B-cell lymphoma (DLBCL). Cloning and characterization of HGAL indicated that it is induced by IL-4. HGAL is a cytoplasmic protein and has been demonstrated in follicular lymphoma, Burkitt lymphoma, lymphocyte-predominant Hodgkin lymphoma, and a subset of DLBCL. HGAL expression is an important predictor of overall survival in patients with germinal center- derived tumors. HGAL protein is also found in germinal centers of normal tonsils and lymph nodes.,Cytoplasm. Cell membrane. Note=It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.,Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL

UniProt: Q8N6F7