Neuroligin 1 antibody (C-Term)

Catalog No. ABIN7233049

This Mouse Monoclonal antibody specifically detects Neuroligin 1 in WB, IHC and IF/ICC. It exhibits reactivity toward Rat.

Quick Overview for Neuroligin 1 antibody (C-Term) (ABIN7233049)

Target: Neuroligin 1 (NLGN1)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neuroligin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S97A-31

Product Details anti-Neuroligin 1 Antibody

Binding Specificity: AA 718-843, C-Term

Purpose: Anti-Neuroligin-1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Neuroligin-1. It does not cross- react with other Neuroligins.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Fusion protein corresponding to aa 718-843 (cytoplasmic C-terminus) of rat Neuroligin- 1, accession no. Q62765). This sequence is 99

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL. A band of ~120 kDa is detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This product is stable for at least one (1) year at -20°C.

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: Nlgn1

Background: Neuroligin-1,Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (By similarity). The protein is involved in nervous system development. {UniProtKB:Q99K10, PubMed:15620359, PubMed:9325340}.,Neuroligin-1 is a neuronal cell surface protein and member of the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses, it participates in regulation of synaptic plasticity, and it is important in the development of long- term memory in adult mammalian amygdala. It's participation in cell-cell interactions may occur through assembly of intracellular junctions by binding of beta-neurexins. In nervous system tissue, Neuroligin-1 is observed primarily in neurons and in the spinal cord.,Cell membrane, Single-pass type I membrane protein. Cell junction, synapse, postsynaptic density, Cell junction, synapse, synaptic cleft, Cell junction, synapse, synaptic cell membrane,Neuroligin I

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis