GPR97 antibody (Extracellular)

Catalog No. ABIN7237675

This Rabbit Polyclonal antibody specifically detects GPR97 in WB, IHC, FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for GPR97 antibody (Extracellular) (ABIN7237675)

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR97 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR97 Antibody

Binding Specificity: AA 62-75, Extracellular

Purpose: A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)

Specificity: Extracellular, N-term

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12 out of 14 amino acid residues identical

Characteristics: Extracellular, N-term

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235457)

Blocking Peptide: (ABIN7235457)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR97

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Alternative Name: ADGRG3 

Background: Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97,Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion-water homeostasis, inflammation and cell-fate determination1.One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2. GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet-induced obesity in mice4.Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Gene ID: 222487

NCBI Accession: NM_170776

UniProt: Q86Y34