Netrin 3 antibody (Secreted)

Catalog No. ABIN7237732

The Rabbit Polyclonal anti-Netrin 3 antibody is suitable to detect Netrin 3 in samples from Mouse. It has been validated for WB and IHC.

Quick Overview for Netrin 3 antibody (Secreted) (ABIN7237732)

Target: Netrin 3 (Ntn3)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Netrin 3 Antibody

Binding Specificity: AA 445-458, Secreted

Purpose: A Rabbit Polyclonal antibody to Netrin-3

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, Human - 13 out of 14 amino acid residues identical

Characteristics: Secreted

Purification: Affinity purified on immobilized antigen.

Immunogen: CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236014)

Blocking Peptide: (ABIN7236014)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Netrin 3

Target: Netrin 3 (Ntn3)

Alternative Name: NTN3

Background:

Synonyms: NTN3, Netrin-2-Like Protein, NTN2L

Description: Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2)1.Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family2.Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas3.

Gene ID: 18209

NCBI Accession: NM_006181

UniProt: Q9R1A3

Pathways: Regulation of Muscle Cell Differentiation