NPX1 antibody (Secreted)

Catalog No. ABIN7237737

This anti-NPX1 antibody is a Rabbit Polyclonal antibody detecting NPX1 in WB and IHC. Suitable for Mouse.

Quick Overview for NPX1 antibody (Secreted) (ABIN7237737)

Target: NPX1 (Neuronal Pentraxin 1 (NPX1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NPX1 Antibody

Binding Specificity: AA 197-211, Secreted

Purpose: A Rabbit Polyclonal antibody to Neuronal Pentraxin 1 (NPTX1)

Specificity: Secreted

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, Human - 13 out of 15 amino acid residues identical

Characteristics: Secreted

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ERAKIESALTSLHQR, corresponding to amino acid residues 197 - 211 of mouse NPTX1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236078)

Blocking Peptide: (ABIN7236078)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPX1

Target: NPX1 (Neuronal Pentraxin 1 (NPX1))

Alternative Name: NPTX1

Background: Neuronal Pentraxin-1, NP1,Neuronal Pentraxin 1 (NP1 or NPTX1) is a 47-50 kDa secreted glycoprotein that is expressed in the central nervous system and involved in excitatory synaptic remodeling1. The pentraxin superfamily members are made from monomers arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain with a shorter inner conserved sequence called the pentraxin signature 2.NP1 is preferentially expressed in the brain and is involved in glutamate receptor internalization. It is secreted pre-synaptically and implicated in excitatory synaptic and mitochondrial deficits. In addition, NP1 may represent a potential synapse-derived plasma biomarker that is able to identify alterations in excitatory synapses and early-onset Alzheimer's disease3.

Alternative names: Neuronal Pentraxin-1, NP1

Gene ID: 18164

NCBI Accession: NM_018713

UniProt: Q62443

Pathways: Carbohydrate Homeostasis, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, SARS-CoV-2 Protein Interactome