NPY1R antibody (2nd Extracellular Loop) (FITC)

Catalog No. ABIN7237741

This Rabbit Polyclonal antibody specifically detects NPY1R in FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for NPY1R antibody (2nd Extracellular Loop) (FITC) (ABIN7237741)

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY1R antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NPY1R Antibody (FITC)

Binding Specificity: 2nd Extracellular Loop, AA 197-210

Purpose: A Rabbit Polyclonal Antibody to NPY1R (extracellular) conjugated to the fluorescent dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: CFDKFPSDSHRLSY, corresponding to amino acid residues 197-210 of mouse NPY1R

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236090)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NPY1R

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Alternative Name: NPY1R

Background:

Synonyms: Neuropeptide Y receptor type 1, NPYR, NPYY1

Description: Neuropeptide Y1 receptor (NPY1R) is one out of four receptors (NPY1R, NPY2R, NPY4R and NPY5R) that bind neuropeptide Y (NPY), an abundant neuropeptide in the mammalian central and peripheral nervous systems.NPY1R is part of the β-branch of class A G-protein coupled receptors and likewise, has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1,2.NPY belongs to a family of gut-brain peptides that demonstrate both hormonal functions by paracrine and endocrine release in the gastrointestinal tract and a neurotransmitter functions in sympathetic ganglia and the CNS. It is highly expressed throughout the brain with particularly high expression in limbic brain areas, such as the hippocampus, amygdala, and hypothalamus3,4. NPY modulates multiple physiological and pathophysiological processes: it increases food intake and reduces fear and anxiety by activating NPY1R1,2.Because of the multiple functions of NPY, NPY1R has great importance in the development and progression of both metabolic and neurological diseases such as diabetes, obesity, breast cancer and bone loss and indeed a number of peptides and small-molecule compounds have been characterized as NPY1R antagonists and have shown clinical potential in the treatment of these pathological states5.

Gene ID: 18166

NCBI Accession: NM_000909

UniProt: Q04573

Pathways: Negative Regulation of Hormone Secretion, Feeding Behaviour