Tetraspanin 3 antibody (Extracellular, Loop 2)

Catalog No. ABIN7886695

The Rabbit Polyclonal anti-Tetraspanin 3 antibody is suitable to detect Tetraspanin 3 in samples from Human. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for Tetraspanin 3 antibody (Extracellular, Loop 2) (ABIN7886695)

Target: Tetraspanin 3 (TSPAN3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Tetraspanin 3 Antibody

Binding Specificity: AA 155-169, Extracellular, Loop 2

Purpose: A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237377)

Blocking Peptide: (ABIN7237377)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Tetraspanin 3

Target: Tetraspanin 3 (TSPAN3)

Alternative Name: TSPAN3

Background:

Synonyms: TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8

Description: Tetraspanin-3, encoded by TSPAN3 gene is a membrane-spanning glycoprotein that has a role in cell migration, proliferation, and interactions between cells and extracellular matrix.As suggested by the family name, TSPANs are categorized by the presence of four trans-membrane domains, of which two are extracellular. The intracellular part includes the C and N terminal domains that can facilitate protein-protein interactions, as well as guide intracellular trafficking. TSPANs have multiple roles In the immune and vascular systems, and they act as organizers of functional microdomains and multi-subunit receptor complexes 1.Tspan3 is highly expressed in the brain and elevated in brains of Alzheimers disease (AD) patients. It was shown to interact with ADAM10, which is a marker for Alzheimer's disease 2. It was also shown to be expressed by dendritic cells (DCs) which suggests that it may be involved in the function of DCs in association with T cell stimulation 3.Its functional diversity and importance for the development and onset of various diseases makes Tetraspanin 3 an appealing research target.

Gene ID: 10099

NCBI Accession: NM_005724

UniProt: O60637