RAF1 antibody (pSer621)

Catalog No. ABIN7271267

The Rabbit Monoclonal anti-RAF1 antibody has been validated for WB. It is suitable to detect RAF1 in samples from Human.

Quick Overview for RAF1 antibody (pSer621) (ABIN7271267)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAF1 Antibody

Binding Specificity: pSer621

Purpose: Phospho-Raf1-S621 Rabbit mAb

Cross-Reactivity: Human, Rat

Characteristics: Phosphorylated Antibodies

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding S621 of human Phospho-Raf1-S621

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],CMD1NN, CRAF, NS5, Raf-1, c-Raf,Apoptosis,Apoptosis_Inhibition of Apoptosis,B Cell Receptor Signaling Pathway,Cancer,Cell Biology & Developmental Biology,Endocrine & Metabolism,ErbB-HER Signaling Pathway,ESC Pluripotency and Differentiation,G protein signaling,G protein signaling_G-Protein-Coupled Receptors Signaling to MAPK/Erk,Growth factors,IL-6 Receptor Signaling Pathway,Immunology & Inflammation,Insulin Receptor Signaling Pathway,Kinase,Kinase_Serine/threonine kinases,MAPK-Erk Signaling Pathway,Mitochondrial metabolism,Mitochondrial metabolism_Mitochondrial markers,Neuroscience,Protein phosphorylation,Signal Transduction,T Cell Receptor Signaling Pathway,RAF1

Molecular Weight: 74kDa

Gene ID: 5894

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling