EZH1 antibody (AA 471-510) (Biotin)

Catalog No. ABIN717628

This Rabbit Polyclonal antibody specifically detects EZH1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EZH1 antibody (AA 471-510) (Biotin) (ABIN717628)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EZH1 Antibody (Biotin)

Binding Specificity: AA 471-510

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EZH1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1

Background:

Synonyms: Enhancer of zeste homolog 1Drosophila, Enhancer of zeste homolog 1, ENX-2, ENX2, EZH1, EZH1: enhancer of zeste homolog 1 Drosophila, EZH1_HUMAN, Histone-lysine N-methyltransferase EZH1, KIAA0388.

Background: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Sequence similarities Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain.

Gene ID: 2145