FCGR2B antibody (Extracellular Domain)

Catalog No. ABIN7271916

The Mouse Monoclonal anti-FCGR2B antibody (Clone 4F5) (ABIN7271916) specifically detects FCGR2B in FACS. The antibody is reactive with Human samples.

Quick Overview for FCGR2B antibody (Extracellular Domain) (ABIN7271916)

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR2B antibody is un-conjugated

Application: Flow Cytometry (FACS)

Grade: GMP Grade

Clone: 4F5

Product Details anti-FCGR2B Antibody

Binding Specificity: Extracellular Domain

Purpose: CD32b Unconjugated Antibody

Characteristics: The clone 4F5, a mouse monoclonal antibody recognizes anti- FcγRIIb, a cell surface protein also known as CD32b. FcγRII has two major isoforms named FcγRIIa and FcγRIIb with high degree of homology. The clone 4F5 selectively reacts with FcγRIIb but not with FcγRIIa. The CD32b antigen is widely expressed on the cell surface of circulating B lymphocytes, monocytes, neutrophils, myeloid dendritic cells (DCs), platelets and at very low levels on plasmacytoid DCs. FcγRs (FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16, and FcγRIV/CD16-2) play important roles in inflammatory cell activation, clearance, presentation of Ag, and maintenance of IgG homeostasis. Published reports claimed that 4F5 partially blocks the ligand-binding of FcRIIb and mediates an inhibitory signal by co-cross-linking FcRIIb with activating FcRs. Clone 4F5 supports study of the expression, regulation, and function of FcγRIIb and may be useful in the area of therapeutic applications where the modulation of FcγRIIb is required.

Purification: Purified

Purity: >95 %

Immunogen: Recombinant human FcgammaRIIb/c (CD32b) extracellular domain

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2, 0.1 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FCGR2B

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: CD32b

Gene ID: 2213

UniProt: P31994

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling