NOS1 antibody

Catalog No. ABIN7272048

This anti-NOS1 antibody is a Mouse Monoclonal antibody detecting NOS1 in WB, IHC, ELISA, ICC and IF. Suitable for Human.

Quick Overview for NOS1 antibody (ABIN7272048)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: J23

Product Details anti-NOS1 Antibody

Purpose: Mouse Anti-Human NOS1 Monoclonal IgG2a

Specificity: Ubiquitously expressed, detected in skeletal muscle and brain, also in testis, lung and kidney.,Detects ~160.97 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Partial Human Recombinant NOS1 Protein

Isotype: IgG2a

Application Details

Application Notes: WB (1:1000), optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution was sufficient for detection of NOS1/Nnos in 7.5 ug of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background: NNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. There are two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II)., cellular localisation: Cell Membrane

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction