tau antibody (AA 297-391) (Atto 594)

Catalog No. ABIN7272117

The Atto 594-conjugated Rabbit Polyclonal anti-tau antibody (ABIN7272117) specifically detects tau in WB. The antibody is reactive with Human samples.

Quick Overview for tau antibody (AA 297-391) (Atto 594) (ABIN7272117)

Target: tau (tau Protein)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This tau antibody is conjugated to Atto 594

Application: Western Blotting (WB)

Product Details anti-tau Antibody (Atto 594)

Binding Specificity: AA 297-391

Purpose: Rabbit Anti-Human Truncated Tau Fragment (AA297-391) (dGAE) Polyclonal,

Specificity: Widely expressed, with high levels in brain.,Detects ~10.2 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A

Immunogen: Recombinant Human Tau AA297-391 (dGAE) Fibril (SPR-461)

Application Details

Application Notes: WB (1:1000), optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution was sufficient for detection of tau in 15 μg of mouse, rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for tau

Target: tau (tau Protein)

Background: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing paired helical filaments (PHFs). A truncated 95-amino acid fragment corresponding to residues 297-391 of full-length tau has been shown to assemble into PHF-like fibrils in vitro in the absence of additives or templates (3). This fragment has been found in the core of PHFs from AD brains and forms filaments that closely resemble PHFs isolated from AD brains (3)., cellular localisation: Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex

Gene ID: 4137

NCBI Accession: NP_005901

UniProt: P10636