Recombinant SARS-CoV-2 Spike S1 antibody (RBD) (Alexa Fluor 647)

Catalog No. ABIN7272864

The Alpaca Monoclonal anti-SARS-CoV-2 Spike S1 antibody has been validated for IF and ICC. It is suitable to detect SARS-CoV-2 Spike S1 in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Spike S1 antibody (RBD) (Alexa Fluor 647) (ABIN7272864)

Target: SARS-CoV-2 Spike S1

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Alpaca

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Spike S1 antibody is conjugated to Alexa Fluor 647

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: W25

Product Details anti-SARS-CoV-2 Spike S1 Antibody (Alexa Fluor 647)

Binding Specificity: RBD

Expression System: E.coli

Specificity: RBD of S1 protein from SARS-CoV-2

Purification: Produced in: E.coli

Immunogen: anti-SARS-CoV-2 S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: IF: 1:1000 (corresponding to 5 nM final concentration)

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details for SARS-CoV-2 Spike S1

Target: SARS-CoV-2 Spike S1

Alternative Name: S1 protein from SARS-CoV-2