Recombinant SLC17A7 antibody (Atto 488)

Catalog No. ABIN7272877

The Atto 488-conjugated Alpaca Monoclonal anti-SLC17A7 antibody (Clone Nb9) (ABIN7272877) specifically detects SLC17A7 in ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant SLC17A7 antibody (Atto 488) (ABIN7272877)

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Human

Host: Alpaca

Clonality: Monoclonal

Conjugate: This SLC17A7 antibody is conjugated to Atto 488

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Nb9

Product Details anti-SLC17A7 Antibody (Atto 488)

Expression System: E.coli

Specificity: Human VGlut1, other species not yet tested, no significant cross-reaction with VGlut2 or VGlut9

Purification: Produced in: E.coli

Immunogen: anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details for SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Alternative Name: VGlut1

Background: The vesicula glutamate transport 1 (vGlut1) is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.