GAPDH antibody (C-Term) (DyLight 488)

Catalog No. ABIN7273056

The Goat Polyclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human, Mouse, Rat, Monkey, Dog and Zebrafish (Danio rerio). It has been validated for WB, IF and IHC (fro).

Quick Overview for GAPDH antibody (C-Term) (DyLight 488) (ABIN7273056)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is conjugated to DyLight 488

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAPDH Antibody (DyLight 488)

Binding Specificity: C-Term

Purpose: Anti-GAPDH, DyLight@488

Sequence: SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

Specificity: Detects a band of 37 kDa by Western blot.

Cross-Reactivity (Details): Reacts against human, rat, mouse, zebrafish, canine and monkey proteins.

Characteristics: Glyceraldehyde-3-phosphate dehydrogenase

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:250, IHC-F:1:200-1:1,000

Restrictions: For Research Use only

Handling

Concentration: 2.5 mg/mL

Buffer: PBS, 20 % glycerol, and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase) conjugated to DyLight® 488. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.