RFP antibody (DyLight 488)

Catalog No. ABIN7273108

The DyLight 488-conjugated Goat Polyclonal anti-RFP antibody (ABIN7273108) specifically detects RFP in WB, IF and IHC (fro). The antibody is reactive with Discosoma and Tomato samples.

Quick Overview for RFP antibody (DyLight 488) (ABIN7273108)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma, Tomato

Host: Goat

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to DyLight 488

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RFP Antibody (DyLight 488)

Purpose: Anti-RFP, DyLight®488

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato.

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Restrictions: For Research Use only

Handling

Concentration: 2.5 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Background:

Goat polyclonal antibody to RFP (Red fluorescent protein) conjugated to DyLight® 488. RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Cherry fluorescent protein, dsRed, red fluorescent protein, tdTomato antibody.