RFP antibody (DyLight 633)

Catalog No. ABIN7273110

The Goat Polyclonal anti-RFP antibody is suitable to detect RFP in samples from Discosoma and Tomato. It has been validated for WB, IF and IHC (fro).

Quick Overview for RFP antibody (DyLight 633) (ABIN7273110)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma, Tomato

Host: Goat

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to DyLight 633

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RFP Antibody (DyLight 633)

Purpose: Anti-RFP, DyLight®633

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato.

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Restrictions: For Research Use only

Handling

Concentration: 2.5 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Background:

Goat polyclonal antibody to RFP (Red fluorescent protein) conjugated to DyLight® 633. RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Cherry fluorescent protein, dsRed, red fluorescent protein, tdTomato antibody.