Vimentin antibody

Catalog No. ABIN7384323

The Rabbit Monoclonal anti-Vimentin antibody has been validated for WB, IF and IHC (p). It is suitable to detect Vimentin in samples from Human, Mouse, Rat and Hamster.

Quick Overview for Vimentin antibody (ABIN7384323)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R02-8C4

Product Details anti-Vimentin Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: CTRCT30,Epididymis luminal protein 113,FLJ36605,HEL113,VIM,VIME,Vimentin,Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA, 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Molecular Weight:

Observed_MW: 54kDa

Calculated_MW: 54kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis