SMAD2 antibody

Catalog No. ABIN7384371

The Rabbit Monoclonal anti-SMAD2 antibody has been validated for WB and IHC (p). It is suitable to detect SMAD2 in samples from Human, Rat and Hamster.

Quick Overview for SMAD2 antibody (ABIN7384371)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Rat, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R03-3F8

Product Details anti-SMAD2 Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000 IHC 1:20-1:100

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: Drosophila,homolog of,MADR2,hMAD-2,HsMAD2,JV18,JV18-1,JV181,MAD,MAD homolog 2,MAD Related Protein 2,Mad-related protein 2,MADH2,MADR2,MGC22139,MGC34440,Mother against DPP homolog 2,Mothers against decapentaplegic homolog 2,Mothers against decapentaplegic,Drosophila,homolog of,2,Mothers against DPP homolog 2,OTTHUMP00000163489,Sma and Mad related protein 2,Sma- and Mad-related protein 2 MAD,SMAD 2,SMAD family member 2,SMAD,mothers against DPP homolog 2,SMAD2,SMAD2,The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Molecular Weight:

Observed_MW: 52kDa

Calculated_MW: 52kDa

Gene ID: 4087

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus