ASK1 antibody

Catalog No. ABIN7384417

This anti-ASK1 antibody is a Rabbit Monoclonal antibody detecting ASK1 in WB. Suitable for Human.

Quick Overview for ASK1 antibody (ABIN7384417)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: R06-4F5

Product Details anti-ASK1 Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: Apoptosis signal regulating kinase 1,Apoptosis signal-regulating kinase 1,ASK 1,ASK-1,ASK1,M3K5,M3K5,MAP/ERK kinase kinase 5,MAP3K5,MAPK/ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen activated protein kinase kinase kinase 5,Mitogen-activated protein kinase kinase kinase 5,Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Molecular Weight:

Observed_MW: 155kDa

Calculated_MW: 155kDa

Gene ID: 4217

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response