E2F1 antibody

Catalog No. ABIN7384438

This anti-E2F1 antibody is a Rabbit Monoclonal antibody detecting E2F1 in WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for E2F1 antibody (ABIN7384438)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R03-5G3

Product Details anti-E2F1 Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000 IHC 1:20-1:100 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: E2F-1,RBAP1,RBBP3,RBP3,E2F1,The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.

Molecular Weight:

Observed_MW: 70kDa

Calculated_MW: 47kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy