Streptavidin antibody

Catalog No. ABIN7384566

The Rabbit Polyclonal anti-Streptavidin antibody has been validated for WB. It is suitable to detect Streptavidin in samples from Various Species.

Quick Overview for Streptavidin antibody (ABIN7384566)

Target: Streptavidin

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Streptavidin Antibody

Purification: Antigen Affinity Purification

Immunogen: Native Streptomyces Avidinii streptavidin protein

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-10000, IP: 3 μg/sample, IF: 1:100-200

Restrictions: For Research Use only

Handling

Concentration: 2.5 mg/mL

Buffer: PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Streptavidin

Target: Streptavidin

Background: Streptavidin, SA, Avidin,Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces. Streptavidin can bind to four moles of biotin per mole of protein with extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.

Molecular Weight:

Observed_MW: 16kDa

Calculated_MW: 16kDa

UniProt: P22629