His Tag antibody (FITC)

Catalog No. ABIN7447733

This Chicken Polyclonal antibody specifically detects His Tag in ELISA, IF and IEP. It exhibits reactivity toward .

Quick Overview for His Tag antibody (FITC) (ABIN7447733)

Target: His Tag

Reactivity: Please inquire

Host: Chicken

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to FITC

Application: ELISA, Immunofluorescence (IF), Immunoelectrophoresis (IEP)

Product Details anti-His Tag Antibody (FITC)

Immunogen: Chickens were immunized with HHHHHH conjugated with KLH (6xHIS) Eggs were collected and the Ig fraction prepared. Antibodies were solid phase adsorbed before immunoaffinity purified using the peptide immobilized on a solid phase matrix then conjugated with fluorescein isothiocyanate (FITC). Antibody concentration was determined using an absorbance at 280 nm: 1.32 equals 1.0 mg of IgY. FITC/Protein Ratio = 3.0 - 6.0.

Application Details

Application Notes: This antibody will react with HHHHHH as determined by ELISA and IEP techniques. It is suitable for blotting, ELISA and IF applications. Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHIS Tag

Target Type: Tag