Rabbits were immunized with highly purified HHHHHH-KLH (6xHIS) and the resulting antiserum was collected. Antibody was solid phase adsorbed, immunoaffinity purified off an antigen containing immunosorbent and conjugated with biotin. Antibody concentration was determined prior to conjugation using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
This antibody reacts with HHHHHH as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.