Rabbits were immunized with highly purified HHHHHH-KLH (6xHIS) and the resulting antiserum was collected. Specific antibody was immunoaffinity purified off an antigen containing immunosorbent and conjugated with fluorescein isothiocyanate (FITC). Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG. FITC/Protein Ratio = 3.0 - 6.0.
This antibody will react with HHHHHH as determined by ELISA and IEP techniques. It is suitable for blotting, ELISA and IF applications. Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.