Recombinant Ki-67 antibody (AA 1200-1250)

Catalog No. ABIN7448915

The Rabbit Monoclonal anti-Ki-67 antibody has been validated for IHC, FACS, ICC, IF/ICC, IF (p) and mIF. It is suitable to detect Ki-67 in samples from Human.

Quick Overview for Recombinant Ki-67 antibody (AA 1200-1250) (ABIN7448915)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (fixed cells) (IF/ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Multiplex Immunofluorescence (mIF)

Clone: BLR021E

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 1200-1250

Purpose: Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [BLR021E]

Immunogen: between AA 1200 and 1250

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:100 - 1:500

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: Background: Ki-67 is a marker of cell proliferation detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in G0. Ki-67 is detected exclusively in the nucleus, and its detection by immunohistological methods has proven to have diagnostic and prognostic value in the study of a variety of human tumors. The specific cellular functions of Ki-67 have not been elucidated, but it is proposed to function in the organization and maintenance of the architecture of DNA and in the synthesis of ribosomes during mitosis.

Gene ID: 4288

NCBI Accession: NP_002408

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process