Apo-B100 antibody (HRP)

Catalog No. ABIN7449329

Product Details anti-Apo-B100 Antibody (HRP)

Target: Apo-B100 (Apoprotein B100 (Apo-B100))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Apo-B100 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Purpose: Goat anti-Human Apo B-100 [H06] Antibody HRP Conjugated

Immunogen: Human Apolipoprotein B100

Clone: H06

Isotype: IgG

Application Details

Application Notes:

WB: 1:1,000 - 1:8,000

ELISA: 1:1,000 - 1:8,000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 50 mM PBS, 0.1 M NaCl, and 0.01 % Thiomersal, pH 7.4

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Apo-B100

Target: Apo-B100 (Apoprotein B100 (Apo-B100))

Alternative Name: ApoB-100 (Apo-B100 Products)

Synonyms: Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, apolipoprotein B antibody, Apob antibody

Background: Background: ApoB exists in human plasma in two isoforms, ApoB-48 (Chen et al., 1987) and Apo B-100 (Wei et al., 1985, Yang et al., 1986a, 1989a,b, 1990, Chen et al., 1986, Yang et al., 1990, Yang and Pownall, 1992). Apo B-100 is the major physiological ligand for the LDL receptor. Apo B-100 is a large monomeric protein, containing 4536 amino acids (m.w. 515 kDa, Yang et al., 1986b). Apo B-100 is synthesized in the liver and is required for the assembly of VLDL. It is found in LDL and VLDL after the removal of the Apo A, E and C. Apo B-48 is present in chylomicrons and their remnants. It is essential for the intestinal absorption of dietary lipids. Apo B levels correlate with the risk of coronary disease. The Apo B protein is directly involved in the retention of LDL with the arterial wall (Olofsson and Boren, 2012). Apo B-48 is synthesized in the small intestine. It comprises approximately half of the N-terminal region of ApoB-100 and is the result of posttranscriptional mRNA editing by a stop codon in the intestine not found in the liver.

Gene ID: 338

NCBI Accession: NP_000375

UniProt: P04114