MCM2 antibody (pSer53)

Catalog No. ABIN7449369

The Rabbit Polyclonal anti-MCM2 antibody (ABIN7449369) specifically detects MCM2 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for MCM2 antibody (pSer53) (ABIN7449369)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MCM2 Antibody

Binding Specificity: pSer53

Purpose: Rabbit anti-Phospho MCM2 (S53) Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: surrounding serine 53

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2

Background: Background: The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.

Gene ID: 4171

NCBI Accession: NP_004517

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA